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Image Search Results
Journal: iScience
Article Title: Patient-derived tumor organoids mimic treatment-induced DNA damage response in glioblastoma.
doi: 10.1016/j.isci.2024.110604
Figure Lengend Snippet: Figure 1. Overview of the procedures used to generate GBOs from resected tumor tissue, cell-type-specific immunostaining of the GBOs and parental tumors and cytokine secretion from GBOs (A) Schematic presentation of main steps for generating GBOs from resected tumor tissue, created with bioRender.com. (B) Growth rate as the quantification of relative area of viable GBOs for five weeks. Data represent mean values GS.E.M. (n = 3 GBOs samples per patient (P)). (C) Immunofluorescence analyses of the GBO TME. GBOs express markers of GSCs (SOX2, CD9, and CD44) and TME cells, such as markers of cells of vasculature (CD31 and CD105), as well as macrophages and microglia (CD68 and Iba1). Representative images from a tissue sample from one patient are shown. Scale bar: 100 mm. (D) Selected cytokines and growth factors are secreted by GBOs in comparison to cultured GB cells from the same patient.
Article Snippet: Antibodies Mouse monoclonal to SOX2 Abcam Cat#ab171380; RRID:AB_2732072 Rabbit polyclonal to GFAP Abcam Cat#ab211271; RRID: N/A Mouse monoclonal to Iba1 Abcam Cat#ab15690; RRID:AB_2224403 Rabbit polyclonal to CD68 Atlas antibodies Cat#HPA048982; RRID:AB_2680587 Mouse monoclonal to CD44 BioRad Cat#MCA2504; RRID:AB_808430 Rabbit monoclonal to CD9 Cell signaling technology Cat#13403; RRID:AB_2732848 Mouse monoclonal to
Techniques: Immunostaining, Comparison, Cell Culture
Journal: PLoS ONE
Article Title: Necl-4/SynCAM-4 Is Expressed in Myelinating Oligodendrocytes but Not Required for Axonal Myelination
doi: 10.1371/journal.pone.0064264
Figure Lengend Snippet: A. Western immuneblotting of brain tissues (from P10 wild-type and Necl-4 homozygous mice) with anti-Necl-1, anti-Necl-2 and anti-Necl-3 antibodies. β-actin was the internal control. B. Statistical analysis on the relative expression level of Necl1, Necl2 and Necl3 with Student's t-test (n = 3. Necl1, p = 0.70. Necl2, p = 0.72. Necl3, p = 0.69). Error bar, standard deviation.
Article Snippet: 30 mg protein from control and mutant tissues was loaded for SDS-PAGE electrophoresis and subsequently detected with anti-Necl-1 (developed in Peking Union Medical University),
Techniques: Western Blot, Expressing, Standard Deviation